Figure 1.

Characterization of retinoblastoma tumor (RBT)- and RB vitreous seeding (RBVS)-derived exosomes. (A) Scanning electron microscopy (SEM) showing a population of heterogeneous-sized exosomes isolated from representative RBT1 and RBVS1 cell lines. Scale bar: 200 nM. A higher magnification image was reported for RBVS1. Scale bar: 100 nM. (B) Graphics representing size distribution of nanoparticles resulting from NanoSight particle-tracking analysis of RBT1 and RBVS1, taken as representative of all RB cell lines. (C) Quantity of total exosomal protein for 10⁶ cells of the different RB cell lines analyzed. (D) Western blot analysis of the typical exosomal proteins, TSG101 and CD9 for two RBT and one RBVS representative cell lines. GAPDH was reported as loading control. Detailed information about western blot can be found at Figure S1.