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. 2020 Jun 19;21(12):4384. doi: 10.3390/ijms21124384

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Experimental scheme for the differentiation of human pluripotent stem cells into dental epithelial-like cells. Mitomycin C (MMC)-treated Hertwig’s epithelial root sheath/epithelial rests of Malassez (HERS/ERM) cells were used as a feeder layer for epithelial-like cell differentiation. Two weeks after the seeding of human induced pluripotent stem cell (hiPSC) clumps on the feeder layer, differentiated epithelial-like cells were collected and characterized. To overcome the limited lifespan of epithelial-like cells, the cells were transfected with an SV40 gene. Immortalized cell lines selected through G418 were characterized by morphology and genetic/immunophenotypic characteristics.