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. 2020 Jun 19;21(12):4384. doi: 10.3390/ijms21124384

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Differentiation of human iPSC (hiPSC) into dental epithelial-like stem cells (EPI-iPSC). (a) Morphological change of hiPSC after differentiation. On day 14, hiPSC had an epithelial cell-like appearance and colony-forming proliferation during subculture. (b) Expression of genes related to the epithelial stem cells in EPI-iPSC after 14 days of induction. EPI-iPSC was positive for E-cadherin, ABCG2, EpCAM, Bmi1, p63, and P75, which are epithelial stem cell markers, and Oct-4, Nanog, and Sox-2, which are stemness-related markers. (c) Fluorescence-activated cell sorting (FACS) analysis of EPI-iPSC. EPI-iPSC was positive for mesenchymal markers (CD29) and HLA type I, but negative for hematopoietic cell markers (CD10, CD45, and HLA-DR) and an endothelial cell marker (CD31). All data were replicated three times.