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. 2020 Jun 19;21(12):4384. doi: 10.3390/ijms21124384

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of the EPI-iPSC cell line on the odontogenic differentiation and mineralization of hdDPSC. EPI-iPSC and hdDPSC were co-cultured with or without a differentiation medium. The hdDPSC and EPI-iPSC alone group was analyzed as a control group. (a) Expression of ameloblast markers (Amelogenin, Enamelin, and KLK4) and odontoblast markers (Runx2, BSP, OCN, DMP1, MEPE, Col1A1, and DSPP) was analyzed by real-time PCR after eight days in culture. (b) Expression patterns of dentin sialoprotein (DSPP) were evaluated by a western blot after 12 days in culture. (c) Mineralized nodule formation of co-culture with EPI-iPSC and hdDPSC in vitro was evaluated by Alizarin red S staining on days 12, 16, and 20. Data are presented as the mean ± SD, n > 3 per group. ^a,b,* p < 0.05. N/D: Not detected.