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. 2020 May 29;12(6):1405. doi: 10.3390/cancers12061405

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inhibition of REST decreased the number of vascular endothelial cells and pericyte coverage in tumor vessels. (A) The endothelial marker CD31 (red) was used to identify tumor vascular structure by immunofluorescence staining. White arrows indicate the punctate lumens. Scale bar: 50 µm. (B) Double staining for the pericyte marker NG2 (green) and endothelial marker CD31 (red) was performed in the different tumor tissues to assess vascular pericytes coverage. NG2 expression and NG2 + CD31 dual-positive vessels were decreased in the R1106 and R1606 tumors compared with the RC-control tumors. White arrows indicate double positive staining (yellow color). Scale bar: 50 µm. (C) Mean NG2 expression in each of the tumor groups was quantified. Bars represent standard deviation. * p < 0.01. (D) The ratio of NG2 to CD31 was calculated in each tumor. Bars represent standard deviation. * p < 0.01.

Inhibition of REST decreased the number of vascular endothelial cells and pericyte coverage in tumor vessels. (A) The endothelial marker CD31 (red) was used to identify tumor vascular structure by immunofluorescence staining. White arrows indicate the punctate lumens. Scale bar: 50 µm. (B) Double staining for the pericyte marker NG2 (green) and endothelial marker CD31 (red) was performed in the different tumor tissues to assess vascular pericytes coverage. NG2 expression and NG2 + CD31 dual-positive vessels were decreased in the R1106 and R1606 tumors compared with the RC-control tumors. White arrows indicate double positive staining (yellow color). Scale bar: 50 µm. (C) Mean NG2 expression in each of the tumor groups was quantified. Bars represent standard deviation. * p < 0.01. (D) The ratio of NG2 to CD31 was calculated in each tumor. Bars represent standard deviation. * p < 0.01.