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. 2020 Jun 1;12(6):1439. doi: 10.3390/cancers12061439

Figure 2.

Figure 2

SPON2 downregulation decreases cell proliferation, migration, and invasion abilities of gastric cancer cells. (A) MKN28 and SNU-601 cell lines were transfected with small interfering RNA (siRNA) specific for SPON2 or scrambled siRNA (scRNA). SPON2 expression levels were detected by RT-PCR and Western blotting. GAPDH was used as loading control. (B) After 48 h following transfection, water soluble tetrazolium salt-1 (WST-1) assays were performed to detect cell proliferation. (C,D) Trans-well cell migration and invasion assays were performed to assess (C) migration and (D) invasion abilities of MKN28 and SNU-601 cells on scRNA or SPON2 siRNA transfection, which are presented in form of graphs (left) and images (right). Data are presented as mean ± standard error of the mean (SEM) (n = 5). p-values were calculated using Student’s t-test, and significant differences are indicated by * (*** p < 0.001).