Figure 6.

Activated Notch1 enhances gastric cancer cell proliferation and motility through upregulation of SPON2. AGS and SNU-601 cells were co-transfected with pcDNA4_caN1 or pcDNA4_dnN1 vector, and SPON2 siRNA or scRNA. (A) mRNA and protein expression levels of N1ICD and SPON2 were detected by RT-PCR and Western blot analyses, respectively. (B) Cell proliferation assay was performed. Results were measured by WST-1 assay after 48 h following transfection. (C) Cell migration (D) and invasion abilities of AGS and SNU-601 cells on scRNA or SPON2 siRNA, and pcDNA4_dnN1 or pcDNA4_caN1 transfection were detected by trans-well cell migration and invasion assays, which are presented in the form of graphs (up) and images (down). Data are presented as mean ± SEM (n = 5). p-values were calculated using Student’s t-test, and significant differences are indicated by * (* p < 0.05, ** p < 0.01, *** p < 0.001).