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. 2020 Jun 1;12(6):1439. doi: 10.3390/cancers12061439

Figure 7.

Figure 7

SPON2 expression levels are regulated on inhibiting Notch signaling pathway in AGS and SNU-601 cells. (A) Cell viability of AGS and SNU-601 cells after 24 h treatment with γ-secretase inhibitor (GSI) (0.1, 0.2, 0.5, 1, 2, 5, and 10 μM) was detected by WST-1 assay. Control was treated with 0.1% dimethyl sulfoxide (DMSO). Data are presented as mean ± SEM (n = 5). p-values were calculated using Student’s t-test, and significant differences are indicated by * (** p < 0.01, *** p < 0.001). (B) mRNA and protein expression levels of SPON2 and N1ICD in AGS and SNU-601 cells on GSI treatment were detected by RT-PCR and Western blot analysis. (C) Schematic model of regulatory mechanism of SPON2 expression through Notch signaling pathway. Activated N1ICD via γ-secretase translocates in nucleus and binds with RBP-Jk in SPON2 promoter region. Subsequently, SPON2 regulates gastric cancer cell proliferation, migration, and invasion.