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. 2020 Jun 22;21(12):4420. doi: 10.3390/ijms21124420

Figure 3.

Figure 3

PKC activation by PMA induces a drop in the number of active TRPM8 channels at the plasma membrane. (A) Dot plot of variance versus mean current obtained from whole-cell currents of a HEK-293 cell stably expressing mTRPM8-myc channels, using 150 ms depolarizing voltage steps from 0 to +180 mV, at 19 °C. Solid line corresponds to fit of data to a parabola (Equation (2)). (B,C) Bar graph representing mean and S.E. and data points of the number of active channels (B) and unitary conductance (C) for cells preincubated for 10 min with 1 µM PMA (blue symbols) or control solution (white symbols). Statistical significance was assessed with a two-tailed unpaired Student’s t test; ** p < 0.01, n = 7 cells for each condition.