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. 2020 Jun 10;12(6):1516. doi: 10.3390/cancers12061516

Figure 7.

Figure 7

Pyridinyl imidazole compounds sensitize human melanoma cells to endoplasmic reticulum (ER) stress. Propidium iodide-based flow cytometry analysis of cell viability treated with small molecule compounds for 48 h. Presented data were obtained in three independent experiments. (A) Viability of BRAF-mutated A375 cells treated with SB202190 (SB; 5, 10, and 15 μM) in combination with ER stressor thapsigargin (T; 50 and 100 nM). (B) Viability of NRAS-mutated MEL-JUSO melanoma cells treated with SB202190 (SB; 5, 10, and 15 μM) in combination with thapsigargin (T; 50 and 100 nM). (C) Viability of A375 cells treated with thapsigargin (T; 50 nM) together with pyridinyl imidazole inhibitors SB202190 (SB202; 15 μM) and SB590885 (SB590; 5 μM), and MEK inhibitor PD184352 (PD; 0.5 μM). (D) Viability of A375 cells treated with SB202190 (SB202; 15 μM) in combination with ER stress inducers thapsigargin (T; 50 nM) and tunicamycin (Tu; 0.5 μM). ** denotes p < 0.01, **** indicates p < 0.0001.