Figure 5.

GAGs from sea cucumber (I. badionotus) with sulfate levels of 4.5% or above have similar anti-inflammatory properties. (a) The sulfate content of freshly-isolated GAGs used in analyses was quantified. The GAGs—LS were derived from the same sample but were reconstituted and lyophilized at least 3 times prior to sulfate analysis. (b) Differences in ear weights (right-left) in mouse ears exposed to vehicle alone (left ear), or inflammatory agent TPA followed by GAGs or GAGs—LS [low sulfate] (right ear). For each figure, n ≥ 4 per group and values with distinct superscripts differ significantly from each other (p ≤ 0.05).