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. 2020 May 29;12(6):1407. doi: 10.3390/cancers12061407

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Schematic outline of the study design. Male C57Bl/6N mice were randomized to one of four groups: (1) the control group (n = 11) was fed a standard diet and tissues were collected after 12 weeks; (2) the non-alcoholic fatty liver (NAFL) group (n = 11) was fed a choline-deficient high-fat (CD-HFD) diet for 12 weeks before tissue collection; (3) the non-alcoholic steatohepatitis (NASH) group (n = 11) received a CD-HFD for 20 weeks and remained sedentary before tissue collection; (4) the NASH + exercise (EXE) group (n = 11) received a CD-HFD for 20 weeks but with treadmill running at 12.5 m/min imposed from weeks 12 to 20.