Figure 11.

Effect of exercise on liver endothelium. (A) Scanning electron microscopy images of sinusoids showing endothelial cells from livers of control, 12-week CD-HFD treated (NAFL), 20-week CD-HFD sedentary (NASH), and 20-week CD-HFD exercised mice (magnification ×15,000) (upper panel). Porosity, defined as the percentage of endothelial cell membrane perforated by fenestrations, was quantified (bottom panel) (unpaired t-test; ***p < 0.001). (B) Immunoblots of the gene product of nitric oxide synthase 3 (NOS3) and its phosphorylated form expressed in liver homogenates of control and NAFL groups (12 weeks treatment), and NASH and NASH + EXE groups (20-week treatment). Immunoblots were quantified and normalized with vinculin. The NAFL, NASH, and NASH + EXE groups are described in Figure 1. For more details of Western blots, please view Figure S2.