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. 2020 May 31;12(6):1434. doi: 10.3390/cancers12061434

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gint4.T-STAT3 and GL21.T-10b combined effect on primary GSC tumour sphere formation. (a,b) Sphere formation of indicated primary GSC-derived tumour spheres (PDGFRβ⁺) left untreated (−) or treated with Gint4.T; Gint4.T-STAT3 or CtrlApt-STAT3; GL21.T-10b; or control aptamers—alone or in combination, as indicated. Left panels: Spheres with a diameter >50 μm were counted and expressed as the percentage relative to the untreated samples (−), set to 100%. Vertical bars depict the mean ± SD (n = 2). Right panels: Boxplot representation of the diameter measures (spheres with a diameter > 25 μm). Statistics of the conjugate treatments versus the untreated samples were obtained using one-way ANOVA: **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.