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. 2020 Jun 13;21(12):4225. doi: 10.3390/ijms21124225

Figure 1.

Figure 1

Sequence and characterization of IRDye 800Cw-conjugated IκB Kinaseγ/NF-κB essential modulator (IKKγ/NEMO)-binding domain (NBD) peptide.(A) Schematic representation of the labeling reaction of the fluorophore dye (IRDye 800Cw NHS Ester) with primary amine groups (Lys) of NBD peptide. Representative high-performance liquid chromatography (HPLC) chromatograms of NBD peptide and (B) IRDye 800Cw-conjugated NBD peptide (C) in PBS; (D) Metabolite profile from mouse urine sample at 4 h after intraperitoneal injection.