Figure 2.

In vivo/ex vivo biodistribution of fluorochrome-conjugated NBD peptide in black and tan brachyuric (BTBR) ob/ob mice. (A) Short-time evaluation of the in vivo biodistribution by three different administration routes; (B) four hours post-injection, ex vivo analysis demonstrates selective kidney metabolization; (C) long-time evaluation of the in vivo biodistribution confirms the presence of fluorophore-conjugated NBD peptide after 48 h of administration. Arrows indicate bladder (white) and fecal (black) excretion. Abbreviations: H—heart; K—kidney; L—liver; E—eye and VF—visceral fat.