Figure 1.

Animal model of preconception maternal weight trajectories. C57BL/6J F0 females were fed a control diet (CD, blue), a high-fat diet (HFD, red) or an HFD for two months and then switched onto the CD. On postnatal day 21 (PND21), half of the litter was weaned onto the HFD and the other half under the paired-CD diet, resulting in six groups: CD-CD, CD-HFD, OB-CD, OB-HFD, WL-CD and WL-HFD. All male offspring were followed until the age of six months. The number of animals studied (n) is indicated for each group.