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. 2020 Jun 13;21(12):4215. doi: 10.3390/ijms21124215

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of 1,25(OH)₂D₃ on Aβ-induced changes in vitamin D receptor (VDR) (a) and glial cell line-derived neurotrophic factor (GDNF) (b) protein expressions. SH-SY5Y cells were incubated with 1 μM Aβ(25-35) prior to the addition of 0.1 and 10 nM 1,25(OH)₂D₃ for 24 h.* Significantly differs from the control group (statistical analysis was performed using Student’s t test). Bars of Aβ, Aβ + 0.1 nM 1,25(OH)₂D₃, and Aβ + 10 nM 1,25(OH)₂D₃ with different letters significantly differ from each other (p < 0.05) (statistical analysis was performed using one-way analysis of variance (ANOVA) with Duncan’s post-hoc analysis). All data are expressed as mean ± SD of three experiments, and each experiment included triplicate repeats.

Effects of 1,25(OH)₂D₃ on Aβ-induced changes in vitamin D receptor (VDR) (a) and glial cell line-derived neurotrophic factor (GDNF) (b) protein expressions. SH-SY5Y cells were incubated with 1 μM Aβ(25-35) prior to the addition of 0.1 and 10 nM 1,25(OH)₂D₃ for 24 h.* Significantly differs from the control group (statistical analysis was performed using Student’s t test). Bars of Aβ, Aβ + 0.1 nM 1,25(OH)₂D₃, and Aβ + 10 nM 1,25(OH)₂D₃ with different letters significantly differ from each other (p < 0.05) (statistical analysis was performed using one-way analysis of variance (ANOVA) with Duncan’s post-hoc analysis). All data are expressed as mean ± SD of three experiments, and each experiment included triplicate repeats.