Skip to main content
. 2020 Jun 14;21(12):4232. doi: 10.3390/ijms21124232

Figure 6.

Figure 6

Amiloride treatment improves neuronal survival after GCI. The presence of live neurons after ischemic insult was confirmed in the Sub, CA1, CA2, and CA3 regions. (A) Fluorescent images show surviving neurons in the Sub, CA1, CA2, and CA3 regions. Intraperitoneal post-treatment with amiloride (10 mg/kg) increased the number of live neurons in the Sub, CA1, CA2, and CA3 regions at 3 days post-ischemia. Scale bar = 20 μm. (B) Bar graph displaying the quantification of surviving neurons in hippocampal subregions. The number of NeuN (+) neurons was increased in the amiloride-injected (10 mg/kg) group in the Sub, CA1, CA2, and CA3 regions compared with the vehicle-treated group (GCI-vehicle, n = 8; GCI-amiloride, n = 6). Data are mean ± SEM. * Considerably different from the vehicle-treated group, p < 0.05; # sham versus vehicle-operated group, p < 0.05. (Kruskal–Wallis test (B) Sub: Chi square = 14.214, df = 3, p = 0.003; CA1: Chi square = 13.422, df = 3, p = 0.004; CA2: Chi square = 14.249, df = 3, p = 0.003; CA3: Chi square = 16.158, df = 3, p = 0.001).