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. 2020 Jun 16;21(12):4295. doi: 10.3390/ijms21124295

Figure 3.

Figure 3

Investigations of leaf development in terrestrial (Tf) and climbing (Cf) forms in Mickelia scandens by anatomical and expression analyses. Longitudinal sagittal sections in (a–c,f); paradermal sections in (d,e). (a) Anatomical section of leaf apex bearing apical cell with a distal lenticular face (black arrowhead) and two dividing proximal cutting faces (their limits pointed out by white arrowheads). Inner derivative cells form the procambium (arrow). In situ hybridization (b–f). (b) MsC1KNOX1 (Tf) and (c) MsC1KNOX2 (Cf) are expressed in the leaf apical cell (arrowhead), derivatives cells, and in procambial strands (ps). (d) MsC1KNOX1 (Tf) and (e) MsC1KNOX2 (Tf) are expressed in young pinnae primordia (arrowheads). (f) MsH4 expression indicates cell division in multiple cells in the apical region and procambial strands (ps). Tf. Bars: (a,b) 50, (c) 200, (d–f) 100 µm.