Table 1.

List of primer pairs used for RT-qPCR analyses of RB cell lines. For relative quantification of mRNA levels, β-Actin (ACTB), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and S18 (RBPS18) served as housekeeping genes. For each gene, the primer sequence and predicted amplicon size is indicated. Abbreviations: bp—base pairs, for—forward, rev—reverse.

Primer Name	Gene	Primer Sequence	Accession Number	Product Size (bp)
Aggrecan for	ACAN	tcaccagtgaggacctcgt	NM_001135.3	84
Aggrecan rev		ggcggtagtggaagacgac	NM_013227.3
β-Actin for	ACTB	ccaaccgcgagaagatga	NM_001101.3	97
β-Actin rev		ccagaggcgtacagggatag
Brevican for	BCAN	tggaagctccactccagaa	NM_021948.4	76
Brevican rev		cggtaccatggattgtgtttc
Collagen IV for	COL4A1	gggagaaaagggtgaagca	NM_001845.5	72
Collagen IV rev		ccaaaggtcctgtgcctataa
Fibronectin for	FN1	cttccacaggaggcctacac	NM_212482.1	114
			NM_002026.2
			NM_212478.1
Fibronectin rev		cgcaaaatatgctggaacttt	NM_212476.1
			NM_212474.1
GAPDH for	GAPDH	tccactggcgtcttcacc	NM_001289746.1	114
			NM_001289745.1
GAPDH rev		ggcagagatgatgaccctttt	NM_001256799.2
			NM_002046.5
α4-Integrin for	ITGA4	gatgaaaatgagcctgaaacg	NM_000885.4	80
α4-Integrin rev		gccatactattgccagtgttga
α5-Integrin for	ITGA5	cccattgaatttgacagcaa	NM_002205.2	92
α5-Integrin rev		tgcaaggacttgtactccaca
β1-Integrin for	ITGB1	cgatgccatcatgcaagt	NM_002211	71
β1-Integrin rev		acaccagcagccgtgtaac	NM_133376
α1-Laminin for	LAMA1	aggatgacctccattctgactt	NM_005559.3	60
α1-Laminin rev		ccttacatgggcactgacct
MMP-2 for	MMP2	ctacgaccgcgacaagaagt	NM_001302510.1	114
			NM_001302509.1
			NM_001302508.1
MMP-2 rev		agttcccaccaacagtggac	NM_001127891.2
			NM_004530.5
MMP-7 for	MMP7	gctgacatcatgattggcttt	NM_002423.3	113
MMP-7 rev		tctcctccgagacctgtcc
MMP-9 for	MMP9	gtaccacggccaactacgac	NM_004994.2	74
MMP-9 rev		gccgtcctgggtgtagagt
Neurocan for	NCAN	aggggttcggagctatgg	NM_004386.2	95
Neurocan rev		ggcccacgtagaagacctc
S18 for	RPS18	cttccacaggaggcctacac	NM_022551.2	82
S18 rev		cgcaaaatatgctggaacttt
TIMP-1 for	TIMP1	agtggcactcattgcttgtg	NM_003254.2	104
TIMP-1 rev		ggactggaagcccttttca
TIMP-2 for	TIMP2	gaagagcctgaaccacaggt	NM_003255.4	85
TIMP-2 rev		cggggaggagatgtagcac
Tenascin-C for	TNC	gtctccagcctgccacag	NM_002160.3	82
Tenascin-C rev		cgcctttccagccaggttc
Tenascin-R for	TNR	agtgacctctgagcccattg	NM_003285.2	61
Tenascin-R rev		gatgtcttttgggggatcaa
Versican for	VCAN	tcccaggaaacttacgatg	NM_004385.4	70
Versican rev		ggggacagtgaggtgggaa