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. 2020 Jun 21;12(6):1643. doi: 10.3390/cancers12061643

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Myoferlin interacts with mitofusins in pancreas cancer cell lines. (A) coimmunoprecipitation of mitofusins and HA-tagged myoferlin with an anti-mitofusin antibody. Western blot of protein samples from whole cells (input), IgG control immunoprecipitation (IgG), and mitofusins immunoprecipitation (anti-MFN1/2) of HA-myoferlin transfected Panc-1 cells. HA-myoferlin and mitofusins were detected on the same membrane; (B) coimmunoprecipitation of mitofusins and endogenous myoferlin with an anti-mitofusin antibody. Western blot of protein samples from whole cells, IgG control immunoprecipitation, and mitofusins immunoprecipitation of Panc-1, BxPC-3, MiaPaCa-2, and PaTu8988T cell lines. Myoferlin and mitofusins were detected on the same membrane; (C) myoferlin (or HA-tagged myoferlin) enrichment in anti-MFN1/2 relatively to IgG. Quantification was performed using ImageJ. All experiments were performed as three independent biological replicates.