Skip to main content
. 2020 Jun 26;12(6):1704. doi: 10.3390/cancers12061704

Figure 2.

Figure 2

Components of non-viral transgene cassette for expression under endogenous promoter: (a) Schematic of non-viral cassette encoding a gene of interest surrounded by a 2A/IRES at the 5′ end for cleavage GOI from endogenous gene, and poly (A) region at the 3′ end for complete termination; LHA, left homology arm; RHA, right homology arm; ∆PAM, mutated PAM sequence of guide (g) RNA. (b) Design of homology arms (HAs) surrounding the cut site of gRNA targeting human TRAC locus. PAM sequence is indicated in the red box. (c) Scheme of the construct used for the study. It consists of IL-15 and mClover3 for dual transgene expression; SA, splice acceptor; P2A, “self-cleaving” 2A peptide derived from porcine teschovirus-1; E2A, “self-cleaving” 2A peptide derived from equine rhinitis A virus; bGH poly (A), bovine growth hormone polyadenylation signal.