Figure 3.

Overview of the steps to generate transgene knock-in in T cell: (a) Molecular steps considering vector design, amplification, purification, and concentration DNA. (b) Electroporation of RNA ribonucleoproteins (RNPs) and donor template to T cells using Lonza instrument. (c) Human T-cell preparation before electroporation. Complete and detailed knock-in protocol can be found in the Supplementary Materials. (d) The 1% agarose gel showing PCR amplicons that were gel purified vs. non-gel purified. (e) Concentration of dsDNA template after purification and concentration (n = 24).