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. 2020 Jun 23;21(12):4454. doi: 10.3390/ijms21124454

Figure 2.

Figure 2

IL6 and IL8 expression and secretion increases upon miR-34a overexpression in HASMCs. HASMCs of 22- or 43-year-old donors were infected with either pMIRNA1 (CTRL) or pMIRH34a (miR-34a) lentivirus. (A) HASMCs were cultured in a growth medium for 48 hour. IL6 expression was quantified by qRT-PCR and normalized to corresponding HPRT levels. Values are mean ± SD; *, p < 0.05; Student’s t-test; n = 3–4. (B) HASMCs were cultured in growth medium for 48 and 72 hours (h). Amount of IL6 was quantified by ELISA assay in the supernatant of cells. Values are mean ± SD; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; 1-way ANOVA followed by Bonferroni’s multiple comparison test; n = 3–4. (C) HASMCs were cultured in growth medium for 48 and 72 h. Amount of IL8 was quantified by ELISA assay in the supernatant of cells. Values are mean ± SD; 22 yo, n = 5; 43 yo, n = 3.