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. 2020 Jun 23;21(12):4454. doi: 10.3390/ijms21124454

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Calcium deposition in Mir34a^+/+ and Mir34a^−/− mice after vitamin D treatment. Twelve-week-old Mir34a^+/+ and Mir34a^−/− mice were treated subcutaneously with either vitamin D (vit D) or a mock solution (Ctrl) for three consecutive days and sacrificed 5 days (Day 5) after the first injection. Calcium content in kidney, lung, heart and aorta (aortic arch) was quantified by the colorimetric analysis. Values are mean ± SD; *, p < 0.05; **, p < 0.01; ****, p < 0.0001; 1-way ANOVA followed by Bonferroni’s multiple comparison test; n = 5, 5, 4 and 5.