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PKCε knockdown inhibits basal autophagy. T47D cells were transfected with control non-targeting or PKCε siRNA. (A) Western blot analysis was performed with indicated antibodies. Tubulin was used to control for loading differences. Bar graph represents mean ± S.E of LC3-II (B) and p62 (C) from several independent experiments. ***, p ≤ 0.0005 (n = 10); **, p ≤ 0.005 (n = 7).
