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. 2020 Jun 15;21(12):4247. doi: 10.3390/ijms21124247

Figure 6.

Figure 6

The effect of PKCε overexpression and mTOR signaling on autophagy. (A) Western blot analysis was performed with MCF-7/Neo and MCF-7/PKCε cells. (B) Bar graph represents mean ± S.E. **, p ≤ 0.005 (n = 10). (C) Effect of Rapamycin on LC3-I, LC3-II and p62. Cells were treated with or without 100 nM Rapamycin and Western blot analysis was performed. (D) MCF-7/Neo and MCF-7/PKCε cells were transfected with control non-targeting, Raptor, Rictor or mTOR siRNA and then treated with or without 10 µM chloroquine for 2 h. Western blot analyses were performed with indicated antibodies. Actin was used to control for loading differences. SE, short exposure; LE-long exposure. The fold change in LC3-II with respect to control and normalized with loading is indicated below the Figures.