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. 2020 May 31;12(6):1431. doi: 10.3390/cancers12061431

Figure 1.

Figure 1

Identification of a three gene signature characteristic to activated myeloid cells. (A) Dendritic cells (DC) and CD14+ monocytes were isolated from healthy donors and stimulated with poly(I:C) (10 µg/mL) or cGAMP (10 μg/mL) for 6 h in vitro. Gene expression counts were quantified using the NanoString nCounter Immunology Panel. Unsupervised t-Distributed Stochastic Neighbor Embedding (t-SNE) analysis showed significant clustering between unstimulated and stimulated cells for both monocytes and DC. (B) IDO1, CXCL11, and GBP1 expression values (RNA-seq, microarray) of unstimulated and stimulated myeloid cells using public Gene Expression Omnibus (GEO) datasets: GSE57494 (LPS + IFNγ), GSE82227 (IFNγ), GSE2706 (LPS), and GSE1925 (IFNγ) plotted as 3D scatter plots using the R package plotly and show clustering between unstimulated and stimulated cells.