Figure 2.

Expression of the myeloid activation (MA) signature genes positively correlated with M1 macrophage and ‘mature DC’ signatures in various cancer types. (A) RNA-seq data from peripheral blood mononuclear cells (PBMC) were obtained from GEO (GSE107011) and analyzed for the expression of the signature genes for every cell type available. Transcripts per million (TPM) values were scored with a non-parametric, rank-based method using the R package singscore based on the co-expression of Cxcl11, Gbp1, and Ido1. (B) Bulk tumor RNA-seq gene expression data was obtained from The Cancer Genome Atlas (TCGA ) cohorts using GDCRNATools in R. The MA signature score was compared to previously described M1 macrophage (upper panel) and mature DC (lower panel) signatures by Pearson correlation using R. X-axis represents TCGA study abbreviations.