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. 2020 Jun 4;12(6):1673. doi: 10.3390/nu12061673

Figure 4.

Figure 4

(a) Relative gene expression of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in non-stimulated PBMCs after overnight treatment with 0.5 mg/mL WGPHs. The data represent the mean of the values calculated with respect to PHA-stimulated, untreated cells with the 2−ΔΔCt method, and the standard error of the mean of each group (n = 39). (b) Enzyme activities of SOD, CAT, GPx, and GR in the supernatants from the same cultures. Activities are expressed as a percentage of the controls. The data represent the mean and standard error of the mean of each group (n = 32). (c) Total glutathione (GSH) content (µM) in non-stimulated PBMCs after overnight treatment with 0.5 mg/mL WGPHs. The data represent the mean of the GSH content and standard error of the mean of each group (n = 28). (d) Total antioxidant capacity (TAC), oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power (FRAP), and trolox equivalent antioxidant capacity (TEAC) quantified on the supernatants from non-stimulated PBMCs cultured overnight with 0.5 mg/mL WGPHs. The data are expressed as a percentage of the control group, and they represent the mean and standard error of the mean of the percentage of each group (n = 32). *** p ≤ 0.001 with respect to the control group (WGPHs 0 mg/mL).