Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Flowchart of the RNA-seq wet-lab and in silico analyses. The main analytic pipeline contains: preprocessing quality control, mapping reads, biological replicates validation, differential genes and long noncoding RNAs (lncRNAs) expression analysis, alternative splicing events identification, allele-specific expression, RNA editing sites and real-time PCR validation. LPS—lipopolysaccharide; SNV—single nucleotide variant; AS—alternative splicing; PCA—principal component analysis.
