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. 2020 May 26;12(6):1363. doi: 10.3390/cancers12061363

Figure 1.

Figure 1

ESM-1 expression levels in MDA-MB-231 cells and their RT-R derivatives. (A,B) ESM-1 mRNA expression in RT-R-MDA-MB-231 cells and their parental cells, MDA-MB-231 was analyzed by RT-PCR. The full blot image can be found in Figure S1. Representative ESM-1 mRNA levels (A) and the quantification of ESM-1 mRNA levels (B) in MDA-MB-231 and RT-R-MDA-MB-231 cells. The data represent the mean ± SD of five independent experiments. (C) Cells were incubated for 72 h, and the levels of ESM-1 secreted by MDA-MB-231 and RT-R-MDA-MB-231 cells into cell culture medium were measured using ELISA kit for ESM-1 as described in the methods sections. The data represent the mean ± SD of five independent experiments. * p < 0.05, ** p < 0.01.