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. 2020 Jun 23;21(12):4451. doi: 10.3390/ijms21124451

Figure 3.

Figure 3

IL-4 ameliorated hypothalamic changes in leptin-deficient mice. Wild-type C57BL/6 (WT) and leptin-deficient 145E mice were fed the normal diet (ND) for 8 weeks. Simultaneously, normal saline and IL-4 (1 μg/mouse) were intraperitoneally administrated twice a week. Total RNAs were extracted from the hypothalamic tissues and subjected to qRT-PCR for the measurement of AgRP (A), NPY (B), POMC (C), TNF-α (F), and IL-1β (G) mRNA expression. Blood samples were collected and subjected to ELISA for the measurement of leptin (D) and ghrelin (E) levels. Proteins were extracted from the hypothalamic tissues and subjected to Western blot analysis with the indicated antibodies. Representative blots and quantitative data are shown (H). * p < 0.05 vs. WT/saline group and # p < 0.05 vs. 145E/saline group, n = 6.