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. 2020 Jun 23;21(12):4451. doi: 10.3390/ijms21124451

Figure 4.

Figure 4

IL-4 ameliorated hepatic changes in leptin-deficient mice. Wild-type C57BL/6 (WT) and leptin-deficient 145E mice were fed the normal diet (ND) for 8 weeks. Simultaneously, normal saline and IL-4 (1 μg/mouse) were intraperitoneally administrated twice a week. Blood samples were collected and GPT (A), GOT (B), total cholesterol (C), and triglycerides (D) measured. Hepatic tissues were collected and subjected to measurement of triglycerides (E). Total RNAs were extracted from the hepatic tissues and subjected to qRT-PCR for the determination of PEPCK (F) and TNF-α (G) mRNA expressions. Proteins were extracted from the hepatic tissues and subjected to Western blot analysis with indicated antibodies. Representative blots and quantitative data are shown (H). * p < 0.05 vs. WT/saline group and # p < 0.05 vs. 145E/saline group, n = 6.