Figure 4.

The effect of CAIX knockdown on cell proliferation. MDA-MB-231 and T47D mock cells (ctrl) and their CAIX KO counterparts were cultured in normoxic or hypoxic (1% O2) conditions. Proliferation was monitored in real-time using the xCELLigence system and expressed as the cell index. Slopes derived from the measurement data indicate the rate of cell growth between time points 5–64 h. The experiment was repeated two times in quadruplicates. p > 0.05 was considered nonsignificant (ns), p < 0.01 is denoted as ** and p < 0.001 as ***.