Table 1.
Cell Culture Media-Reagents and their Respective Concentrations.
| Medium | Reagent | Supplier | Cat.No. |
|---|---|---|---|
| TSC: DMEM complete |
Dulbecco’s modified Eagle Medium | Thermo Fisher Scientific 1 | 61965026 |
| 10% Fetal Bovine Serum | Sigma-Aldrich 2 | F7524 | |
| 2% HEPES Buffer solution | Biochrom 3 | L1613 | |
| 1% non-essential amino acids | Biochrom 3 | K 0293 | |
| 1% sodium pyruvate | VWR 4 | L0473 | |
| 1% Penicillin/Streptomycin | Biochrom 3 | A2213 | |
| OBSC: Dissection buffer | Hibernate A | Thermo Fisher Scientific 1 | A1247501 |
| 1× Glutamax | Thermo Fisher Scientific 1 | 35050061 | |
| 1× Antibiotic-Antimycotic | Thermo Fisher Scientific 1 | 15240062 | |
| 1× B27 | Thermo Fisher Scientific 1 | 17504044 | |
| OBSC: Cultivation medium | Neurobasal A | Thermo Fisher Scientific 1 | 10888022 |
| 1× Glutamax | Thermo Fisher Scientific 1 | 35050061 | |
| 1× Antibiotic-Antimycotic | Thermo Fisher Scientific 1 | 15240062 | |
| 1× B27 | Thermo Fisher Scientific 1 | 17504044 |
1 Thermo Fisher Scientific, Waltham, MA, USA; 2 Sigma-Aldrich, Missouri, MI, USA; 3 Biochrom, Berlin, Germany; 4 VWR, Radnor, PA, USA.