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. 2020 Jun 12;12(6):1547. doi: 10.3390/cancers12061547

Figure 1.

Figure 1

Indoleamine 2,3-dioxygenase 1 (IDO1) is upregulated in cervical cancer stem cells (CSCs) and irradiated cervical cancer cells. (A) The expression levels of IDO1 mRNA among normal cervical or cervical cancer tissues were analyzed by the Gene Expression Profiling Interactive Analysis (GEPIA) website using the data of the Cancer Genome Atlas (TCGA). * p < 0.01. (B, C) The total cell proteins were harvested from HeLa and SiHa cells under 2-dimensional culture (2D) or tumorsphere culture (S). The IDO1 protein expression was determined by Western blotting (B). The inserted numbers indicated the relative expression level of S in comparison to 2D. The IDO1 activity was determined by the conversion of kynurenine from tryptophan using Ehlrich reagent and measured the absorbance at 492 nm (C). The data were presented as fold change to 2D group. (D) The total cell proteins were harvested from parental (P) or irradiated (R) HeLa and SiHa cells and the IDO1 protein expression was determined by Western blotting. The inserted numbers indicate the relative expression level of R in comparison to P. (E) The culture supernatant of parental (P) or irradiated (R) HeLa and SiHa cells was mixed with RPMI-1640 medium at a ratio of 1:1 and used for examining the T cell suppression effect by measuring the proliferation of Jurkat T cells. The control indicated the mixture of fresh DMEM medium and RPMI-1640 medium.