Figure 3.

Involvement of C/EBP homologous protein (CHOP) in DWP05195-induced apoptosis. (A) A2780 cells were treated with DWP05195 (15 µM) for the indicated time and CHOP protein levels were detected using Western blotting. Uncropped blots of Figure 3A are shown in Figure S1 (B) A2780 cells were treated with DWP05195 for 24 h at the indicated concentrations and CHOP mRNA levels were determined by real-time RT-PCR. * p < 0.05 compared with control. (C) CHOP mRNA and protein levels after transfection with CHOP siRNA in A2780 cells were measured by real-time RT-PCR and Western blotting, respectively. Uncropped blots of Figure 3C are shown in Figure S1 (D) After the CHOP knockdown, cells were treated with 15 µM DWP05195 and used for analysis of apoptosis. Results are representative of at least three independent experiments. * p < 0.05.