Figure 8.

Involvement of NADPH oxidase activation in DWP05195-induced apoptosis. (A) A2780 cells were transfected with p47phox siRNA for 24 h and treated with DWP05195 (15 µM) for 2 h. The cells were stained with DCFH-DA and analyzed by flow cytometry. (B) After knockdown of p47phox, cells were treated with DWP05195 (15 µM) for 24 h. CHOP levels were measured by real-time RT-PCR. (C) After knockdown of p47phox, cells were treated with DWP05195 (15 µM). MTT assay was performed to determine cell viability. Results are representative of at least three independent experiments. * p < 0.05.