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. 2020 Jun 3;12(6):1451. doi: 10.3390/cancers12061451

Figure 3.

Figure 3

Modulation of RKIP expression in primary melanoma cell lines. (a) RKIP mRNA levels in RKIP-downregulated A375 and MelHO primary melanoma cell lines. A375 and MelHO cells were transduced with RKIP shRNA Lentiviral Particles or Control shRNA Lentiviral Particles following the manufacturer´s instruction. Two days after infection, the cells were selected with Puromycin to get stable cell lines; (b) Western Blot assay showed the RKIP-downregulation in A375 and MelHO melanoma cells; (c) Proliferation rate in A375 and MelHO primary melanomas after RKIP downregulation. The viability of control melanoma cells (with an empty vector) and stable RKIP transfected clones were subjected to XTT assays for 24, 48, and 72 h. Results of each experiment are expressed related to the values obtained for the transfection control. Data is given as a mean ± SD of at least three experiments of different transfection; (d) Fold change on wound healing rate in A375 and MelHO primary melanoma after RKIP downregulation; (e) Fold change on active migration rate in presence of collagen in primary melanoma after RKIP downregulation. The histograms in (d) and (e) show the average of three independent assays with six replicates per assay and representative pictures have been included. * p-value < 0.05.