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. 2020 Jun 3;12(6):1451. doi: 10.3390/cancers12061451

Figure 4.

Figure 4

Modulation of RKIP expression in MeWO and A2058 metastatic melanoma cell lines. (a) RKIP mRNA levels in RKIP-upregulated MeWO and A2058 metastatic melanoma cell lines. A2058 and MeWO cell lines were transfected with overexpressing plasmid for RKIP (pRKIP) or empty plasmid (pCTR) using Lipofectamine 2000 as transfection agent. All of the transfection experiments were performed with 500 ng of DNA. (b) Western Blot assay showed the RKIP-upregulation in MeWO and A2058 melanoma cells; (c) Fold change on wound healing rate in MeWO and A2058 metastatic melanoma after RKIP upregulation. The experimental assays were performed at least after 24 h of RKIP transfection; (d) Fold change on active migration rate in presence of collagen in metastatic melanoma after RKIP upregulation. The histograms in (c) and (d) show the average of three independent assays with six replicates per assay and representative pictures have been included. * p-value < 0.05.