Table 4.

Targeting of ATM, ATR and DNA-Pkcs alone and in combination with photons and protons to decrease 3D HPV-negative HNSCC spheroid growth.

Inhibitor	FaDu	A253
ATM	p = 0.69	p = 0.49
ATR	p = 0.89	p = 0.72
DNA-Pkcs	p = 0.82	p = 0.88
ATM + photons	p = 0.09	p < 0.002
ATR + photons	p = 0.28	p < 0.003
DNA-Pkcs + photons	p < 0.003	p < 0.002
ATM + protons	p < 0.03	p < 0.006
ATR + protons	p = 0.24	p = 0.11
DNA-Pkcs + protons	p < 0.005	p < 0.002

Statistical analysis was performed on the dataset across the 15-day growth period using a one-way ANOVA, comparing the growth of inhibitor treated spheroids against the appropriate DMSO control (± radiation).