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. 2020 Jun 3;12(6):1663. doi: 10.3390/nu12061663

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Pentadecanoic acid suppresses the stem like-cell characteristics of MCF-7/SC. (a) Effects of pentadecanoic acid on MCF-7/SC mammosphere formation (magnification 100×). (b,b’) The effects of pentadecanoic acid on the expression of cell surface markers in MCF-7/SC were analyzed by FACS analysis following pentadecanoic acid exposure. (c) Flow cytometry analysis of the ALDH⁺ population using the ALDEFLUOR assay kit. Cells were treated with pentadecanoic acid for 48 h prior to the assay. (d) The levels of cancer stem cell (CSC) markers were assessed by Western blot experiments following pentadecanoic acid treatment for 48 h. (e) The levels of CSC markers as assessed by Western blot experiments following 100 μM pentadecanoic acid exposure. GAPDH was used as a loading control. Data are shown as the mean ± standard deviation of three biologically independent experiments. * p < 0.05 vs. control.

Pentadecanoic acid suppresses the stem like-cell characteristics of MCF-7/SC. (a) Effects of pentadecanoic acid on MCF-7/SC mammosphere formation (magnification 100×). (b,b’) The effects of pentadecanoic acid on the expression of cell surface markers in MCF-7/SC were analyzed by FACS analysis following pentadecanoic acid exposure. (c) Flow cytometry analysis of the ALDH⁺ population using the ALDEFLUOR assay kit. Cells were treated with pentadecanoic acid for 48 h prior to the assay. (d) The levels of cancer stem cell (CSC) markers were assessed by Western blot experiments following pentadecanoic acid treatment for 48 h. (e) The levels of CSC markers as assessed by Western blot experiments following 100 μM pentadecanoic acid exposure. GAPDH was used as a loading control. Data are shown as the mean ± standard deviation of three biologically independent experiments. * p < 0.05 vs. control.