Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 27;12(6):1371. doi: 10.3390/cancers12061371

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Sex-dependent metabolic alteration in OS patients with primary tumors (PT) and lung metastases (LM). (A) Heat map for the unsupervised hierarchical clustering of the n = 7 PT and n = 5 LM (males = M and females = F). Perseus version 1.4.0.20 was used for clustering. The Z-score from the normalized peak areas from the technical and biological replicates for the annotated metabolites from the central carbon metabolism was calculated. Negative Z-scores were displayed in green, positive Z-Scores in purple. An unweighted average linkage clustering and Euclidean distance preprocessed with k-means was used for the hierarchical clustering. (B) Levels of annotated metabolites of glycolysis, the TCA cycle, glycerol and amino acids in n = 7 PT and n = 5 LM from males and females. Data show the mean and standard deviation of the normalized peak area. Samples were analyzed using an unpaired Student’s t-test, with a p ≤ 0.05 deemed as statistically significant and indicated by the star (*). 2HG: 2-hydroxy-glutaric acid. 2PGA: glyceric-acid-2-phosphate. 3PGA: glyceric-acid-3-phosphate. 13BPG: 1/3-bis-phosphoglyceric acid. aCoA: acetyl-CoA. Ala: alanine. aKG: alpha-ketoglutaric acid. Asn: asparagine. Asp: aspartic acid. Cit: citric acid. Cys: cysteine. DHAP: dihydroxyacetonephosphate. F6P: fructose-6-phosphate. F16BP: fructose-1/6-bisphopsphate. Fum: fumaric acid. G6P: glucose-6-phosphate. GA3P: glyceraldehyde-3-phosphate. Glc: glucose. Gln: glutamine. Glu: glutamic acid. Glyc3P: glycerol-3-phosphate. Gly: glycine. Glyc: glycerol. Ile: isoleucine. Lac: lactic acid. Leu: leucine. Lys: lysine. Mal: malic acid. Met: methionine. OAA: oxaloacetate. Phe: phenylalanine. PEP: phosphoenol-pyruvic acid. Pyr: pyruvic acid. Pro: proline. Ser: serine. Suc: succinic acid. TCA: tricarboxylic acid. Thr: threonine. Trp: tryptophan. Tyr: tyrosine. Val: valine.

Sex-dependent metabolic alteration in OS patients with primary tumors (PT) and lung metastases (LM). (A) Heat map for the unsupervised hierarchical clustering of the n = 7 PT and n = 5 LM (males = M and females = F). Perseus version 1.4.0.20 was used for clustering. The Z-score from the normalized peak areas from the technical and biological replicates for the annotated metabolites from the central carbon metabolism was calculated. Negative Z-scores were displayed in green, positive Z-Scores in purple. An unweighted average linkage clustering and Euclidean distance preprocessed with k-means was used for the hierarchical clustering. (B) Levels of annotated metabolites of glycolysis, the TCA cycle, glycerol and amino acids in n = 7 PT and n = 5 LM from males and females. Data show the mean and standard deviation of the normalized peak area. Samples were analyzed using an unpaired Student’s t-test, with a p ≤ 0.05 deemed as statistically significant and indicated by the star (*). 2HG: 2-hydroxy-glutaric acid. 2PGA: glyceric-acid-2-phosphate. 3PGA: glyceric-acid-3-phosphate. 13BPG: 1/3-bis-phosphoglyceric acid. aCoA: acetyl-CoA. Ala: alanine. aKG: alpha-ketoglutaric acid. Asn: asparagine. Asp: aspartic acid. Cit: citric acid. Cys: cysteine. DHAP: dihydroxyacetonephosphate. F6P: fructose-6-phosphate. F16BP: fructose-1/6-bisphopsphate. Fum: fumaric acid. G6P: glucose-6-phosphate. GA3P: glyceraldehyde-3-phosphate. Glc: glucose. Gln: glutamine. Glu: glutamic acid. Glyc3P: glycerol-3-phosphate. Gly: glycine. Glyc: glycerol. Ile: isoleucine. Lac: lactic acid. Leu: leucine. Lys: lysine. Mal: malic acid. Met: methionine. OAA: oxaloacetate. Phe: phenylalanine. PEP: phosphoenol-pyruvic acid. Pyr: pyruvic acid. Pro: proline. Ser: serine. Suc: succinic acid. TCA: tricarboxylic acid. Thr: threonine. Trp: tryptophan. Tyr: tyrosine. Val: valine.