Figure 1.
Cell death assessment in myeloid leukemia cell lines treated with SR59230A in hypoxia (1% O2) and in normoxia (21% O2). (A) Apoptosis evaluation through Annexin V and Propidium Iodide in HEL, K562, KCL22 and HL60 cell lines treated with different concentrations of SR59230A (1 μM, 3 μM, 6 μM, 8 μM, 10 μM) for 24 h, in normoxia and in hypoxia; (B) apoptosis evaluation through Annexin V and Propidium Iodide in HEL, K562, KCL22 and HL60 cell lines treated with different concentrations of SR59230A (1 μM, 3 μM, 6 μM, 8 μM, 10 μM) for 48 h, in normoxia and in hypoxia. Significance was calculated by one-way ANOVA analysis followed by Bonferroni’s post-hoc test. Results are reported as mean ± SD of three independent experiments performed in duplicate. n = 6 per group. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 SR vs. Ctrl normoxia; ° P < 0.05, °°° P < 0.001, °°°° P < 0.0001 SR vs. Ctrl hypoxia).