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. 2020 Jun 12;21(12):4210. doi: 10.3390/ijms21124210

Figure 2.

Figure 2

Evaluation of beta3-adrenoreceptor (β3-AR)-positive cells and β3-AR/Annexin V-positive cells in myeloid leukemia cell lines under hypoxia (1% O2) and normoxia (21% O2) for 48 h. (A) β3-AR-positive cells in HEL, K562, KCL22 and HL60 cell lines in normoxia and hypoxia; significance was calculated by unpaired T-test in each cell line. Results are reported as mean ± SD of four independent experiments. n = 4 per group (** P < 0.01, **** P < 0.0001 hypoxia vs. normoxia). (B) Flow cytometric analysis of HEL, K562, KCL22 and HL60 cell lines in control and SR59230A 5 µM treated cells in normoxia and hypoxia. Annexin V-positive cells gated on a β3-AR-positive cells subpopulation are showed. Significance was calculated by unpaired T-test in each cell lines. Results are reported as mean ± SD of four independent experiments. n = 4 per group. (** P < 0.01, **** P < 0.0001 SR hypoxia vs. SR normoxia).