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. 2020 Jun 26;12(6):1699. doi: 10.3390/cancers12061699

Table 1.

Comparison of oncolytic virus vectors.

Adenovirus Herpes Simplex Virus Vaccinia Virus Reovirus
Genome dsDNA dsDNA dsDNA Segmented dsRNA
Capsid Icosahedral Icosahedral Complex icosahedral
Virion diameter 70–90 nm 150–200 nm 170–200 nm 60–100 nm
Genome size 36–40 kb 150–200 kb 130–280 kb 0.2–3 kb
Replication site Nucleus Nucleus Cytoplasm Cytoplasm
Life cycle 24 h 12 h 8 h 18 h
Immunogenicity High Moderate High Low
Transgene expression Transient Potentially long-term Transient Transgene expression is not common
Ease of manipulation Easy Difficult Easy Very difficult
Cloning capacity 8 kb >30 kb 25 kb n/a
Easily achievable titers 1012 PFU/mL 1010 PFU/mL 109 PFU/mL 109 PFU/mL
Maximum Dose used in human 6 × 1012 VP [31] 4 × 108 PFU [32] 3 × 109 PFU [26] 3 × 1010 TCID50 [33]
Virulence of WT virus Yes Mild Mild No

dsDNA, Double stranded DNA; dsRNA, Double stranded RNA; kb, kilobase; PFU, plaque forming unit; VP, virus particle; TCID50, median tissue culture infectious dose.