Figure 6.

Produce of H₂O₂ by CAP-treatment and its influence on apoptosis. Ultrapure water was treated with CAP for 5, 10, 20, 40, and 80 s (A). H₂O₂ concentrations were determined with Ample Red Hydrogenperoxid/Peroxidase-Assay-Kit. Representative of a human OS cell line MNNG/HOS (B) and a human ES cell line A673 (C) were treated with 100 µM H₂O₂. After 24 h and 48 h, the activity of the caspases 3 and 7 was measured. As positive control (+) staurosporine was used, (−) are unlabeled cells (B, C). Data show mean ± SD, significant differences were indicated by using a paired t-test as follows: ** p ≤ 0.01.