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. 2020 Jun 16;12(6):1590. doi: 10.3390/cancers12061590

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cancer-associated fibroblasts (CAFs) increased the oncogenic properties of colon cancer cells. (A) Insert illustrates the co-culture system of DLD1 and HCT116 colorectal cancer (CRC) cells with Normal fibroblasts (NF) and CAFs. Cell viability assay showed increased 5-fluorouracil (5-FU) resistance in CAF-educated DLD1 and HCT116 cells, compared to their naïve counterparts. Flow cytometric analysis of DLD1 and HCT116 cells co-cultured with CAFs. Increased CD44⁺ cell population (B) and side population (C) in both DLD1 and HCT116 cells, 48 h post CAF culture. Enhanced colony-forming (D), migratory (E), and tumor sphere-generating (F) abilities in both CRC cell lines post CAF co-culture. ** p < 0.01, *** p < 0.001.